ANGI-11. RAB27 IS REQUIRED FOR REGULATING INTACT VASCULATURE IN GLIOBLASTOMA

Abstract Rab27 is a GTPase involved in the biogenesis of extracellular vesicles (EVs) and cellular secretion pathway. Among two isoforms (a b) Rab27a has been identified regulating angiogenesis during development wound healing via VEGFR1. Here, we show that plays role stability brain vasculature including pathological glioblastoma.. Thus, relative to wild-type C57bl/6 (WT) double heterozygous Rab27a+/-;Rab27b+/- (dHET) mice, animals with Rab27a/b knock-out (Rab27a/b-dKO) exhibit reduced microvascular density cortices. Additionally, also observed reduction permeability Evans Blue dKO cortices controls. When neoangiogensis was induced following orthotopic inoculation mouse glioma cells (GL261), similar morphological anomalies were tumour tumours established control mice. Moreover, vascular Rab27a/b-dKO mice markedly exceeded wild type or Our results suggest Rab27b may play critical ensuring under physiological conditions glioma.